ARG63041

anti-Insulin antibody [IN-05]

anti-Insulin antibody [IN-05] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Radioimmunoassay,Functional study and Human,Bovine,Pig

Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [IN-05] recognizes Insulin
Tested Reactivity Hu, Bov, Pig
Tested Application ELISA, FuncSt, ICC/IF, IHC-P, RIA
Specificity The clone IN-05 reacts with insulin, one of the major regulatory endocrine hormones of intermediate metabolism, normally secreted by the beta cells (a type of islet cells) of the pancreas; it is also present in tumors of B cell origin such as insulinoma.
Host Mouse
Clonality Monoclonal
Clone IN-05
Isotype IgG1
Target Name Insulin
Antigen Species Pig
Immunogen Porcine insulin.
Conjugation Un-conjugated
Alternate Names IDDM; IDDM2; IDDM1; ILPR; MODY10; Insulin; IRDN

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-PAssay-dependent
RIAAssay-dependent
Application Note Functional studies: The clone IN-05 blocks binding of insulin to the receptor.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3630 Human INS

GeneID: 397415 Pig INS

Swiss-port # P01308 Human Insulin

Swiss-port # P01315 Pig Insulin

Gene Symbol INS
Gene Full Name insulin
Background Insulin and glucagon are pancreatic endocrine hormones secreted by islet cells within the pancreas. The stimulus for insulin secretion is a HIGH blood glucose. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population.
Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 12 kDa

Clone References

Transforming growth factor beta is a critical regulator of adult human islet plasticity.

IHC-P / Human

Hanley S et al.
Mol Endocrinol.,  (2007)

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Murine hybridoma monoclonal antibodies against insulin: cross-reactivity with insulins of three species and blocking of insulin binding to its receptor.

Horejsí V et al.
Immunol Lett.,  (1984)

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