ARG22707

anti-Integrin alpha E2 antibody [OX-62] (PE)

anti-Integrin alpha E2 antibody [OX-62] (PE) for Flow cytometry and Rat

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [OX-62] recognizes Integrin alpha E2
This antibody recognizes OX-62 antigen, shown to be Rat alpha E2 integrin which is expressed by dendritic cells of dendritic epidermal T cells, intraepithelial lymphocytes in the small intestine and by cells of dendritic morphology in lymphoid organs, at sites where gamma delta T cells are present.
Tested Reactivity Rat
Tested Application FACS
Host Mouse
Clonality Monoclonal
Clone OX-62
Isotype IgG1
Target Name Integrin alpha E2
Antigen Species Rat
Immunogen Density gradient enriched PVG rat thoracic duct dendritic cells.
Conjugation PE
Alternate Names CD103; HUMINAE; Integrin alpha-E; Mucosal lymphocyte 1 antigen; CD antigen CD103; HML-1 antigen; Integrin alpha-IEL

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose.
Preservative 0.09% Sodium azide
Stabilizer 1% BSA and 5% Sucrose
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol ITGAE
Gene Full Name integrin, alpha E
Background Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. [provided by RefSeq, Jul 2008]
Function Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. [UniProt]
Calculated MW 130 kDa