ARG63043
anti-Intra Acrosomal Protein antibody [Hs-14]
anti-Intra Acrosomal Protein antibody [Hs-14] for Flow cytometry,Western blot,ICC/IF and Human,Mouse
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [Hs-14] recognizes Intra Acrosomal Protein |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, WB |
| Specificity | The clone Hs-14 reacts with a 220 kDa testis-specific human intra-acrosomal protein associated with the membranes of the acrosomal vesicle._x000D_ |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | Hs-14 |
| Isotype | IgM |
| Target Name | Intra Acrosomal Protein |
| Antigen Species | Human |
| Immunogen | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
| Conjugation | Un-conjugated |
| Alternate Names | D11S4365; Acrosomal vesicle protein 1; SP-10; Acrosomal protein SP-10; SPACA2 |
Application Instructions
| Application Suggestion |
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| Application Note | ICC/IF: Staining technique: Membrane permeabilization (acetone) is essential. Application note: The clone Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by precipitation methods and size-exclusion chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACRV1 |
| Gene Full Name | acrosomal vesicle protein 1 |
| Background | One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction. |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody |
| Calculated MW | 28 kDa |
Clone References
Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis.
[Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization].
