ARG23310

anti-JAM-C antibody [CRAM-18 F26] (FITC)

anti-JAM-C antibody [CRAM-18 F26] (FITC) for Flow cytometry,ICC/IF and Mouse,Human

Overview

Product Description FITC-conjugated Rat Monoclonal antibody [CRAM-18 F26] recognizes JAM-C
Rat anti Mouse JAM-c antibody, clone CRAM-18 F26 recognises mouse and human Junctional adhesion molecule C (JAM-C), also known as JAM-3 and, historically, as JAM-2. JAM-C is expressed at junctions between endothelial and epithelial cells, as well as on leukocytes, platelets, vascular smooth muscle cells and fibroblasts, amongst other cell types. It plays a role in tight junctions and inflammatory processes and interacts with JAM-A and JAM-B. Clone CRAM-18 F26 has been reported to inhibit transendothelial migration (Johnson-Léger et al. 2002).
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF
Host Rat
Clonality Monoclonal
Clone CRAM-18 F26
Isotype IgG2a
Target Name JAM-C
Antigen Species Mouse
Immunogen Recombinant soluble JAM-C.
Conjugation FITC
Alternate Names JAM-2; JAM-3; Junctional adhesion molecule C; JAM-C; Junctional adhesion molecule 3; JAMC

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:25
ICC/IFAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.09% Sodium azide and 1% BSA.
Preservative 0.09% Sodium azide
Stabilizer 1% BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 83700 Human JAM3

GeneID: 83964 Mouse JAM3

Swiss-port # Q9BX67 Human Junctional adhesion molecule C

Swiss-port # Q9D8B7 Mouse Junctional adhesion molecule C

Gene Symbol JAM3
Gene Full Name junctional adhesion molecule 3
Background Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]
Function Participates in cell-cell adhesion. It is a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis. [UniProt]
Calculated MW 35 kDa
PTM Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17; the release of soluble JAM3 is increased by proinflammatory factors. [UniProt]