ARG63322

anti-JIP3 / Syd 2 / JSAP1 antibody

anti-JIP3 / Syd 2 / JSAP1 antibody for IHC-Formalin-fixed paraffin-embedded sections and Mouse

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes JIP3 / Syd 2 / JSAP1
Tested Reactivity Ms
Predict Reactivity Hu, Rat, Cow
Tested Application IHC-P
Specificity This antibody is expected to recognise both reported Human isoforms of this protein (as represented by NP_055948.2 and NP_001035529.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name JIP3 / Syd 2 / JSAP1
Antigen Species Human
Immunogen MEIQMDEGGGVV-C
Conjugation Un-conjugated
Alternate Names JIP-3; Mitogen-activated protein kinase 8-interacting protein 3; syd; JIP3; JNK MAP kinase scaffold protein 3; JNK-interacting protein 3; JSAP1; C-Jun-amino-terminal kinase-interacting protein 3; SYD2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P4 - 6 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 30957 Mouse MAPK8IP3

Swiss-port # Q9ESN9 Mouse C-Jun-amino-terminal kinase-interacting protein 3

Background The protein encoded by this gene shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies of the similar gene in mouse suggested that this protein may interact with, and regulate the activity of numerous protein kinases of the JNK signaling pathway, and thus function as a scaffold protein in neuronal cells. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
Research Area Signaling Transduction antibody
Calculated MW 147 kDa
PTM Phosphorylation by ROCK1 is crucial for the recruitment of JNK.