ARG55526
anti-K-Ras antibody
anti-K-Ras antibody for Flow cytometry,Western blot and Human,Mouse
Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes K-Ras |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | FACS, WB |
| Specificity | Based on the sequence homologous information, this antibody might cross-react to H-Ras (70%) and N-Ras (90%). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | K-Ras |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 146-174 (C-terminus) of Human K-Ras. |
| Conjugation | Un-conjugated |
| Alternate Names | NS3; KRAS1; KRAS2; RALD; K-RAS4A; GTPase KRas; c-Ki-ras; KI-RAS; RASK2; K-RAS2B; K-RAS2A; K-RAS4B; c-K-ras; C-K-RAS; NS; CFC2; Ki-Ras; K-Ras 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | NIH/3T3 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRAS |
| Gene Full Name | Kirsten rat sarcoma viral oncogene homolog |
| Background | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008] |
| Function | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. [UniProt] |
| Research Area | Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 22 kDa |
| PTM | Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). |
