ARG66655

anti-KAT7 / HBO1 / MYST2 antibody

anti-KAT7 / HBO1 / MYST2 antibody for Western blot,ICC/IF,ChIP,Immunoprecipitation and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes KAT7 / HBO1 / MYST2
Tested Reactivity Hu, Ms
Tested Application ChIP, ICC/IF, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name KAT7 / HBO1 / MYST2
Antigen Species Human
Immunogen Synthetic peptide within aa. 100-180 of Human KAT7 / HBO1 / MYST2.
Conjugation Un-conjugated
Alternate Names EC 2.3.1.48; ZC2HC7; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2; HBOA; Lysine acetyltransferase 7; Histone acetyltransferase KAT7; MYST-2; HBO1; MYST2; Histone acetyltransferase binding to ORC1

Application Instructions

Application Suggestion
Tested Application Dilution
ChIPAssay-dependent
ICC/IF1:200 - 1:1000
IPAssay-dependent
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 75 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11143 Human KAT7

GeneID: 217127 Mouse KAT7

Swiss-port # O95251 Human Histone acetyltransferase KAT7

Swiss-port # Q5SVQ0 Mouse Histone acetyltransferase KAT7

Gene Symbol KAT7
Gene Full Name K(lysine) acetyltransferase 7
Function Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription. [UniProt]
Cellular Localization Nucleus, nucleoplasm. Cytoplasm, cytosol. Nucleus. Note=Associates with replication origins specifically during the G1 phase of the cell cycle (PubMed:18832067). [UniProt]
Calculated MW 71 kDa
PTM Phosphorylation at Ser-57 by PLK1 during mitosis seems important for prereplicative complex formation and DNA replication licensing, and requires prior phosphorylation at Thr-85 and Thr-88 by CDK1 (PubMed:18250300).

Autoacetylation at Lys-432 is required for proper function. [UniProt]