ARG42195
anti-KCNN4 / KCa3.1 antibody
anti-KCNN4 / KCa3.1 antibody for Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes KCNN4 / KCa3.1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | KCNN4 / KCa3.1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to K309-Q364 of Human KCNN4 / KCa3.1. |
| Conjugation | Un-conjugated |
| Alternate Names | SK4; IK1; KCa3.1; SKCa 4; KCA4; hKCa4; SKCa4; IKCa1; hSK4; IK; Intermediate conductance calcium-activated potassium channel protein 4; hIKCa1; Putative Gardos channel; IKCA1; KCa4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Caco-2, PC-3, A549, HeLa, Rat stomach, Rat testis, Mouse testis and Mouse liver | ||||
| Observed Size | ~ 48 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15554 Human Intermediate conductance calcium-activated potassium channel protein 4 Swiss-port # O89109 Mouse Intermediate conductance calcium-activated potassium channel protein 4 |
|---|---|
| Gene Symbol | KCNN4 |
| Gene Full Name | potassium channel, calcium activated intermediate/small conductance subfamily N alpha, member 4 |
| Background | The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily. [provided by RefSeq, Jul 2008] |
| Function | Forms a voltage-independent potassium channel that is activated by intracellular calcium (PubMed:26148990). Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells (PubMed:17157250, PubMed:18796614). Plays a role in the late stages of EGF-induced macropinocytosis (PubMed:24591580). [UniProt] |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. [UniProt] |
| Calculated MW | 48 kDa |
| PTM | Phosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel. [UniProt] |
