ARG53984
anti-KDM1 / LSD1 antibody
anti-KDM1 / LSD1 antibody for Western blot,ICC/IF,Immunoprecipitation and Human,Monkey
Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes KDM1A |
|---|---|
| Tested Reactivity | Hu, Mk |
| Tested Application | ICC/IF, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | KDM1 / LSD1 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human KDM1/LSD1 protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | Flavin-containing amine oxidase domain-containing protein 2; Lysine-specific histone demethylase 1A; EC 1.-.-.-; BRAF35-HDAC complex protein BHC110; AOF2; KDM1; LSD1; BHC110 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 110 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 3 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O60341 Human Lysine-specific histone demethylase 1A |
|---|---|
| Gene Symbol | KDM1A |
| Gene Full Name | lysine (K)-specific demethylase 1A |
| Background | Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3,thereby acting as a coactivator or a corepressor,depending on the context.Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.Acts as a corepressor by mediating demethylation of H3K4me,a specific tag for epigenetic transcriptional activation.Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me.May play a role in the repression of neuronal genes.Alone,it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity.Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription,by being recruited to ANDR target genes and mediating demethylation of H3K9me,a specific tag for epigenetic transcriptional repression.The presence of PRKCB in ANDR-containing complexes,which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph),a specific tag that prevents demethylation H3K4me,prevents H3K4me demethylase activity of KDM1A.Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation.Demethylates and stabilizes the DNA methylase DNMT1.Required for gastrulation during embryogenesis.Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses,via histone deacetylase (HDAC) recruitment,a number of genes implicated in multilineage blood cell development.Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1,CDN7 and KRT8.Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7. |
| Function | Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7. [UniProt] |
| Cellular Localization | Nucleus |
| Research Area | Gene Regulation antibody |
| Calculated MW | 93 kDa |
| PTM | Polyubiquitinated by JADE2; which leads to its proteasomal degradation. |
