ARG40753

anti-KDM7A / JHDM1D antibody

anti-KDM7A / JHDM1D antibody for Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes KDM7A / JHDM1D
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name KDM7A / JHDM1D
Antigen Species Mouse
Immunogen Recombinant fusion protein corresponding to aa. 417-735 of Mouse KDM7A (NP_001028602.2).
Conjugation Un-conjugated
Alternate Names EC 1.14.11.-; Lysine-specific demethylase 7A; Lysine-specific demethylase 7; JHDM1D; JmjC domain-containing histone demethylation protein 1D

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse spleen
Observed Size 107 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 338523 Mouse KDM7A

GeneID: 80853 Human KDM7A

Swiss-port # Q3UWM4 Mouse Lysine-specific demethylase 7A

Swiss-port # Q6ZMT4 Human Lysine-specific demethylase 7A

Gene Symbol KDM7A
Gene Full Name lysine (K)-specific demethylase 7A
Function Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate. [UniProt]
Cellular Localization Nucleus. [UniProt]
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Calculated MW 107 kDa