ARG41205
anti-KIAA0101 antibody
anti-KIAA0101 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes KIAA0101 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | KIAA0101 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-111 of Human KIAA0101 (NP_055551.1). |
| Conjugation | Un-conjugated |
| Alternate Names | NS5ATP9; p15PAF; OEATC1; p15/PAF; PCNA-associated factor of 15 kDa; PCNA-associated factor; L5; p15(PAF); HCV NS5A-transactivated protein 9; PAF; OEATC-1; Overexpressed in anaplastic thyroid carcinoma 1; PAF15; OEATC; Hepatitis C virus NS5A-transactivated protein 9 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | HT-29 | ||||
| Observed Size | 12 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KIAA0101 |
| Gene Full Name | KIAA0101 |
| Function | PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm, perinuclear region. Note=Following DNA damage, localizes to DNA damage sites (PubMed:21628590). Colocalizes with centrosomes in perinuclear region (PubMed:21673012). [UniProt] |
| Calculated MW | 12 kDa |
| PTM | Monoubiquitinated at Lys-15 and Lys-24 during normal S phase, promoting its association with PCNA. Also diubiquitinated at these 2 sites. Following DNA damage, monoubiquitin chains at Lys-15 and Lys-24 are probably extended, leading to disrupt the interaction with PCNA. Polyubiquitinated by the APC/C complex at the mitotic exit, leading to its degradation by the proteasome. [UniProt] |
