ARG43051

anti-KIF15 antibody

anti-KIF15 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes KIF15
Tested Reactivity Hu, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name KIF15
Antigen Species Human
Immunogen Recombinant protein corresponding to M1201-S1388 of Human KIF15.
Conjugation Un-conjugated
Alternate Names NY-BR-62; Kinesin-like protein 2; Serologically defined breast cancer antigen NY-BR-62; KNSL7; Kinesin-like protein KIF15; Kinesin-like protein 7; HKLP2; hKLP2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 353302 Rat KIF15

GeneID: 56992 Human KIF15

Swiss-port # Q7TSP2 Rat Kinesin-like protein KIF15

Swiss-port # Q9NS87 Human Kinesin-like protein KIF15

Gene Symbol KIF15
Gene Full Name kinesin family member 15
Function Plus-end directed kinesin-like motor enzyme involved in mitotic spindle assembly. [UniProt]
Cellular Localization Cytoplasm. Cytoskeleton, spindle. Note=Detected during the interphase in the cytoplasm as finely punctuate pattern and irregularly shaped dots. Detected during mitosis on the mitotic spindle. Colocalizes with TPX2 in mitosis. Localizes at the central spindle at anaphase. Localizes at the sites of invaginating cell membranes, a position that corresponds to the location of the contractile actomyosin ring of dividing cells. Colocalizes with actin in interphase. [UniProt]
Calculated MW 160 kDa