ARG63050
anti-KIF5B / Kinesin Heavy Chain antibody [KN-03]
anti-KIF5B / Kinesin Heavy Chain antibody [KN-03] for ICC/IF and Human,Mouse,Rat,Pig
Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [KN-03] recognizes KIF5B / Kinesin Heavy Chain |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Tested Application | ICC/IF |
| Specificity | The clone KN-03 recognizes heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. KN-03 well recognizes kinesin bound to taxol-stabilized microtubules._x000D_ |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | KN-03 |
| Isotype | IgM |
| Target Name | KIF5B / Kinesin Heavy Chain |
| Antigen Species | Pig |
| Immunogen | Enriched fraction of porcine brain kinesin. |
| Conjugation | Un-conjugated |
| Alternate Names | HEL-S-61; Kinesin-1 heavy chain; Conventional kinesin heavy chain; KINH; KNS1; Ubiquitous kinesin heavy chain; UKHC; KNS |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by thiophilic adsorption-affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KIF5B |
| Gene Full Name | kinesin family member 5B |
| Background | Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function. |
| Research Area | Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 110 kDa |
