ARG54751
anti-KMT2E / MLL5 antibody
anti-KMT2E / MLL5 antibody for Western blot and Human
Cancer antibody; Developmental Biology antibody; Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes KMT2E / MLL5 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | KMT2E / MLL5 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 93-120 (N-terminus) of Human MLL5 (NP_061152.3). |
| Conjugation | Un-conjugated |
| Alternate Names | Lysine N-methyltransferase 2E; NKp44L; EC 2.1.1.43; MLL5; Histone-lysine N-methyltransferase 2E; HDCMC04P; Myeloid/lymphoid or mixed-lineage leukemia protein 5 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | HL-60 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8IZD2 Human Histone-lysine N-methyltransferase 2E |
|---|---|
| Gene Symbol | KMT2E |
| Gene Full Name | lysine (K)-specific methyltransferase 2E |
| Background | This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a protein with an N-terminal PHD zinc finger and a central SET domain. Overexpression of the protein inhibits cell cycle progression. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008] |
| Function | Histone methyltransferase that specifically mono- and dimethylates 'Lys-4' of histone H3 (H3K4me1 and H3K4me2). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Plays an essential role in retinoic-acid-induced granulopoiesis by acting as a coactivator of RAR-alpha (RARA) in target gene promoters. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. Required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Overexpression inhibits cell cycle progression, while knockdown induces cell cycle arrest at both the G1 and G2/M phases. Isoform NKp44L: Cellular ligand for NCR2/NKp44, may play a role as a danger signal in cytotoxicity and NK-cell-mediated innate immunity. [UniProt] |
| Cellular Localization | Nucleus speckle. Note=Absent from the nucleolus |
| Research Area | Cancer antibody; Developmental Biology antibody; Gene Regulation antibody |
| Calculated MW | 205 kDa |
| PTM | O-glycosylation at Thr-440 in the SET domain by OGT is essential for the histone methyltransferase and the coactivator activity toward RARA in granulopoiesis. The absence of Thr-440 glycosylation in assays done in vitro may explain why some authors did not detected any histone methyltransferase activity for this protein. |
