ARG54749
anti-KMT3A / SETD2 antibody
anti-KMT3A / SETD2 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes KMT3A / SETD2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | KMT3A / SETD2 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 21-50 (N-terminus) of Human KMT3A / SETD2 (NP_054878.5). |
| Conjugation | Un-conjugated |
| Alternate Names | HIF-1; SET domain-containing protein 2; SET2; Huntingtin-interacting protein B; Huntingtin yeast partner B; KMT3A; Huntingtin-interacting protein 1; hSET2; EC 2.1.1.43; HBP231; Lysine N-methyltransferase 3A; HIP-1; p231HBP; HSPC069; Histone-lysine N-methyltransferase SETD2; HYPB |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9BYW2 Human Histone-lysine N-methyltransferase SETD2 |
|---|---|
| Gene Symbol | SETD2 |
| Gene Full Name | SET domain containing 2 |
| Background | Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II. [provided by RefSeq, Aug 2008] |
| Function | Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression. [UniProt] |
| Cellular Localization | Nucleus. Chromosome |
| Research Area | Gene Regulation antibody |
| Calculated MW | 288 kDa |
| PTM | May be automethylated. |
