ARG43799
anti-KMT4 / Dot1L antibody
anti-KMT4 / Dot1L antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes KMT4 / Dot1L |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | KMT4 / Dot1L |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to Human KMT4 / Dot1L. |
| Conjugation | Un-conjugated |
| Protein Full Name | Histone-lysine N-methyltransferase, H3 lysine-79 specific |
| Alternate Names | KMT4; DOT1; Histone-lysine N-methyltransferase, H3 lysine-79 specific; H3-K79-HMTase; DOT1-like protein; Lysine N-methyltransferase 4; EC 2.1.1.43; Histone H3-K79 methyltransferase |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa; Mouse testis | ||||||
| Observed Size | ~180 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 50 nM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Concentration | Batch dependent |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8TEK3 Human Histone-lysine N-methyltransferase, H3 lysine-79 specific |
|---|---|
| Gene Symbol | DOT1L |
| Gene Full Name | DOT1-like histone H3K79 methyltransferase |
| Background | The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011] |
| Function | Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 165 kDa |
| PTM | Phosphoprotein |
