ARG43799

anti-KMT4 / Dot1L antibody

anti-KMT4 / Dot1L antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes KMT4 / Dot1L
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name KMT4 / Dot1L
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to Human KMT4 / Dot1L.
Conjugation Un-conjugated
Protein Full Name Histone-lysine N-methyltransferase, H3 lysine-79 specific
Alternate Names KMT4; DOT1; Histone-lysine N-methyltransferase, H3 lysine-79 specific; H3-K79-HMTase; DOT1-like protein; Lysine N-methyltransferase 4; EC 2.1.1.43; Histone H3-K79 methyltransferase

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa; Mouse testis
Observed Size ~180 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 nM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Concentration Batch dependent
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 84444 Human DOT1L

Swiss-port # Q8TEK3 Human Histone-lysine N-methyltransferase, H3 lysine-79 specific

Gene Symbol DOT1L
Gene Full Name DOT1-like histone H3K79 methyltransferase
Background The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]
Function Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 165 kDa
PTM Phosphoprotein