ARG63985

anti-KPNB1 antibody

anti-KPNB1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Controls and Markers antibody; Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes KPNB1
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name KPNB1
Antigen Species Human
Immunogen C-RRSKTNKAKTLAT
Conjugation Un-conjugated
Alternate Names Pore targeting complex 97 kDa subunit; Importin-90; Importin subunit beta-1; IPOB; Nuclear factor p97; PTAC97; Impnb; Karyopherin subunit beta-1; NTF97; IPO1; IMB1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.03 - 0.1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3837 Human KPNB1

Swiss-port # Q14974 Human Importin subunit beta-1

Background Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq, Jul 2008]
Research Area Controls and Markers antibody; Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 97 kDa
PTM Mono-ADP-ribosylated by PARP16.