ARG11112

anti-Ki-67 antibody

anti-Ki-67 antibody for Western blot,ICC/IF and Human,Mouse,Rat

Overview

Product Description Chicken Polyclonal antibody recognizes Ki-67
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Chicken
Clonality Polyclonal
Isotype IgY
Target Name Ki-67
Antigen Species Human
Immunogen Mixture of two recombinant Human Ki-67 constructs: aa. 1-300 and aa. 1111-1490.
Conjugation Un-conjugated
Alternate Names Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000 - 1:5000
WB1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 5 mM Sodium azide.
Preservative 5 mM Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4288 Human MKI67

Swiss-port # P46013 Human Antigen KI-67

Gene Symbol MKI67
Gene Full Name marker of proliferation Ki-67
Background This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
Function Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). [UniProt]
Cellular Localization Chromosome. Nucleus. Nucleus, nucleolus. Note=Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). [UniProt]
Calculated MW 359 kDa
PTM Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. [UniProt]