ARG11112
anti-Ki-67 antibody
anti-Ki-67 antibody for Western blot,ICC/IF and Human,Mouse,Rat
Overview
Product Description | Chicken Polyclonal antibody recognizes Ki-67 |
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Tested Reactivity | Hu, Ms, Rat |
Tested Application | ICC/IF, WB |
Host | Chicken |
Clonality | Polyclonal |
Isotype | IgY |
Target Name | Ki-67 |
Antigen Species | Human |
Immunogen | Mixture of two recombinant Human Ki-67 constructs: aa. 1-300 and aa. 1111-1490. |
Conjugation | Un-conjugated |
Alternate Names | Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Buffer | PBS and 5 mM Sodium azide. |
Preservative | 5 mM Sodium azide |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | MKI67 |
Gene Full Name | marker of proliferation Ki-67 |
Background | This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009] |
Function | Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). [UniProt] |
Cellular Localization | Chromosome. Nucleus. Nucleus, nucleolus. Note=Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). [UniProt] |
Calculated MW | 359 kDa |
PTM | Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. [UniProt] |