ARG62365
anti-Ki-67 antibody [B126.1]
anti-Ki-67 antibody [B126.1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human,Marmoset
Microvascular Density Study antibody; Proliferation Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [B126.1] recognizes Ki-67 |
|---|---|
| Tested Reactivity | Hu, Marmoset |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | B126.1 |
| Isotype | IgG1 |
| Target Name | Ki-67 |
| Antigen Species | Human |
| Immunogen | raised against nuclear fractions of human tumor cell line |
| Conjugation | Un-conjugated |
| Alternate Names | Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105 |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified Antibody |
| Buffer | 1X PBS and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Ki-67 |
| Gene Full Name | marker of proliferation Ki-67 |
| Background | Ki-67 is a nuclear protein. It is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009] |
| Function | Ki-67 required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). [UniProt] |
| Research Area | Microvascular Density Study antibody; Proliferation Marker antibody |
| Calculated MW | 359 kDa |
| PTM | Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. |
Clone References
A combinatorial microRNA therapeutics approach to suppressing non-small cell lung cancer.
Expressional alterations in functional ultra-conserved non-coding RNAs in response to all-trans retinoic acid--induced differentiation in neuroblastoma cells.
WB / Human
Prickle1 is expressed in distinct cell populations of the central nervous system and contributes to neuronal morphogenesis.
Regulation of retinal progenitor expansion by Frizzled receptors: implications for microphthalmia and retinal coloboma.
IHC / Mouse
Dasatinib sensitizes KRAS mutant colorectal tumors to cetuximab.
IHC-P / Human
Comparative immunohistochemical staining of atherosclerotic plaques using F16, F8 and L19: Three clinical-grade fully human antibodies.
IHC-Fr / Human
