ARG53222
anti-Ki-67 antibody [SP6]
anti-Ki-67 antibody [SP6] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Western blot and Human,Marmoset,Mouse,Rat,Zebrafish
Microvascular Density Study antibody; Proliferation Marker antibody
Overview
Product Description | Rabbit Monoclonal antibody [SP6] recognizes Ki-67 |
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Tested Reactivity | Hu, Ms, Rat, Marmoset, Zfsh |
Tested Application | FACS, ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB |
Host | Rabbit |
Clonality | Monoclonal |
Clone | SP6 |
Isotype | IgG |
Target Name | Ki-67 |
Antigen Species | Human |
Immunogen | Synthetic peptide from C-terminus of human Ki-67 protein. |
Conjugation | Un-conjugated |
Alternate Names | Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105 |
Application Instructions
Application Suggestion |
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Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Positive Control | Tonsil |
Properties
Form | Liquid |
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Buffer | Tissue culture supernatant in TBS (pH 7.5), 1% BSA and < 0.1% Sodium azide |
Preservative | < 0.1% Sodium azide |
Stabilizer | 1% BSA |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Background | Ki-67 is a nuclear protein, which is expressed in the proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein. |
Cellular Localization | Nucleus |
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Research Area | Microvascular Density Study antibody; Proliferation Marker antibody |
Calculated MW | 345 kDa and 395 kDa |
PTM | Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. |
Customer's Feedback
Excellent
Review for anti-Ki-67 antibody [SP6]
Application:IF/ICC
Sample:HeLa
Fixation Buffer:100% Methanol
Fixation Time:10 min
Fixation Temperature:RT ºC
Permeabilization Buffer:0.1% Triton X-100
Primary Antibody Dilution Factor:1:200
Primary Antibody Incubation Time:overnight
Primary Antibody Incubation Temperature:4 ºC
Conjugation of Secondary Antibody:TRITC
Excellent
Review for anti-Ki-67 antibody [SP6]
Application:IF/ICC
Sample:HeLa
Fixation Buffer:100% Methanol
Fixation Time:10 min
Fixation Temperature:RT ºC
Permeabilization Buffer:0.1% Triton X-100
Primary Antibody Dilution Factor:1:200
Primary Antibody Incubation Time:overnight
Primary Antibody Incubation Temperature:4 ºC
Conjugation of Secondary Antibody:TRITC
Specific References