ARG53222

anti-Ki-67 antibody [SP6]

anti-Ki-67 antibody [SP6] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Western blot and Human,Marmoset,Mouse,Rat,Zebrafish

Microvascular Density Study antibody; Proliferation Marker antibody
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publication_link Publication21

Overview

Product Description

Rabbit Monoclonal antibody [SP6] recognizes Ki-67

Tested Reactivity Hu, Ms, Rat, Marmoset, Zfsh
Tested Application FACS, ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SP6
Isotype IgG
Target Name Ki-67
Antigen Species Human
Immunogen Synthetic peptide from C-terminus of human Ki-67 protein.
Conjugation Un-conjugated
Alternate Names Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IF1:200-1:500
IHC-FoFr Assay-dependent
IHC-Fr1:200-1:1000
IHC-P1:200
WB1:200-1:500
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Tonsil

Properties

Form Liquid
Buffer Tissue culture supernatant in TBS (pH 7.5), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4288 Human MKI67

Swiss-port # P46013 Human Antigen KI-67

Background Ki-67 is a nuclear protein, which is expressed in the proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein.
Cellular Localization Nucleus
Highlight Related products:
Ki67 antibodies; Ki67 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Research Area Microvascular Density Study antibody; Proliferation Marker antibody
Calculated MW 345 kDa and 395 kDa
PTM Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.

Customer's Feedback

nuts_pic      Excellent

Review for anti-Ki-67 antibody [SP6]

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:TRITC

nuts_pic      Excellent

Review for anti-Ki-67 antibody [SP6]

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:TRITC

nuts_pic      Good

Review for anti-Ki-67 antibody [SP6]

Application:WB

Sample:HeLa

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

KLF15 suppresses tumor growth and metastasis in Triple-Negative Breast Cancer by downregulating CCL2 and CCL7

IHC-P / Human

Quist Kanyomse et al.
Sci Rep,  (2022)

publication_link

 

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Zinc-finger protein 382 antagonises CDC25A and ZEB1 signaling pathway in breast cancer

WB / Human

Shuman Li et al.
Genes & Diseases,  (2022)

publication_link

 

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Circular RNA circ_0010235 sponges miR-338-3p to play oncogenic role in proliferation, migration and invasion of non-small-cell lung cancer cells through modulating KIF2A.

IHC-P / Human

Yanan Zhu et al.
Ann Med.,  (2021)

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Developing a nomogram for predicting intravesical recurrence after radical nephroureterectomy: a retrospective cohort study of mainland Chinese patients.

IHC-P / Human

Shicong Lai et al.
Jpn J Clin Oncol.,  (2021)

publication_link

 

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Tacrolimus inhibits oral carcinogenesis through cell cycle control.

IHC-P / Human

Yuanyuan Li et al.
Biomed Pharmacother.,  (2021)

publication_link

 

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A bivalent cyclic RGD-siRNA conjugate enhances the antitumor effect of apatinib via co-inhibiting VEGFR2 in non-small cell lung cancer xenografts.

IHC-P / Human

Lumin Liao et al.
Drug Deliv.,  (2021)

publication_link

 

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Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma.

IHC-P / Human

Qinchao Hu et al.
Cell Death Dis.,  (2020)

publication_link

 

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Long-Term Liraglutide Administration Induces Pancreas Neogenesis in Adult T2DM Mice.

IHC-P / Mouse

Deng Hongjun et al.
Cell Transplant.,  (2020)

publication_link

 

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A bubble bursting-mediated oral drug delivery system that enables concurrent delivery of lipophilic and hydrophilic chemotherapeutics for treating pancreatic tumors in rats.

IHC-P / Rat

Chen Kuan-Hung et al.
Biomaterials.,  (2020)

publication_link

 

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Sight and switch off: Nerve density visualization for interventions targeting nerves in prostate cancer.

IHC-P / Mouse

You Huijuan et al.
Sci Adv.,  (2020)

publication_link

 

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CSN6 expression is associated with pancreatic cancer progression and predicts poor prognosis.

IHC-P / Human

Shi Jiaqi et al.
Cancer Biol Ther.,  (2019)

publication_link

 

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Monitoring the Early Antiproliferative Effect of the Analgesic-Antitumor Peptide, BmK AGAP on Breast Cancer Using Intravoxel Incoherent Motion With a Reduced Distribution of Four b-Values.

WB, IHC-P / Mouse

Doudou NR et al.
Front Physiol.,  (2019)

publication_link

 

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ITGB4 deficiency induces senescence of airway epithelial cells through p53 activation.

IHC-P / Mouse

Yuan Lin et al.
FEBS J.,  (2019)

publication_link

 

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ZMYND10, an epigenetically regulated tumor suppressor, exerts tumor-suppressive functions via miR145-5p/NEDD9 axis in breast cancer.

IHC-P / Human

Wang Yan et al.
Clin Epigenetics.,  (2019)

publication_link

 

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The linc00152 Controls Cell Cycle Progression by Regulating CCND1 in 16HBE Cells Malignantly Transformed by Cigarette Smoke Extract.

IHC-P / Mouse

Liu Zhenzhong et al.
Toxicol Sci.,  (2018)

publication_link

 

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An efficient bivalent cyclic RGD-PIK3CB siRNA conjugate for specific targeted therapy against glioblastoma in vitro and in vivo.

IHC-P / Human

Cen Bohong et al.
Molecular Therapy: Nucleic Acid.,  (2018)

publication_link

 

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IL-33 facilitates proliferation of colorectal cancer dependent on COX2/PGE2

IHC-P / Mouse

Li Yongkui et al.
J Exp Clin Cancer Res.,  (2018)

publication_link

 

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CHAC2 is essential for self-renewal and glutathione maintenance in human embryonic stem cells.

FACS / Human

Wang Cheng-Kai et al.
Free Radic Biol Med.,  (2017)

publication_link

 

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Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF‑β1‑mediated cell cycle arrest.

IHC-P / Human

Bu Shi-Xia et al.
International Journal of Oncology.,  (2017)

publication_link

 

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Lymphotoxin-beta interacts with methylated EGFR to mediate acquired resistance to cetuximab in head and neck cancer.

IHC / Zebrafish

Hsu DS et al.
Clin Cancer Res.,  (2017)

publication_link

 

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Human endometrial mesenchymal stem cells exhibit intrinsic anti-tumor properties on human epithelial ovarian cancer cells.

IHC-P / Human

Bu S et al.
Scientific Reports, 6(37019),  (2016)

publication_link

 

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