ARG62528

anti-Ku 70 / Ku 80 antibody [162]

anti-Ku 70 / Ku 80 antibody [162] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Monkey,Mouse,Rat,Xenopus laevis

Cancer antibody; Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody [162] recognizes Ku 70 / Ku 80
Tested Reactivity Hu, Ms, Rat, Mk, Xenopus laevis
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Clone 162
Isotype IgG2a
Target Name Ku 70 / Ku 80
Antigen Species Human
Immunogen Human B cell nuclei from plasmacytoid 2p68 cells
Conjugation Un-conjugated
Alternate Names DNA repair protein XRCC6; Thyroid-lupus autoantigen; Lupus Ku autoantigen protein p70; EC 4.2.99.-; EC 3.6.4.-; ATP-dependent DNA helicase II 70 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 6; CTC box-binding factor 75 kDa subunit; 70 kDa subunit of Ku antigen; CTC75; 5'-deoxyribose-5-phosphate lyase Ku70; KU70; TLAA; 5'-dRP lyase Ku70; CTCBF; ML8; G22P1; X-ray repair cross-complementing protein 6; ATP-dependent DNA helicase 2 subunit 1; Ku70

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa cells and tonsil tissue.

Properties

Form Liquid
Purification Purified Antibody
Buffer 1X PBS and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14375 Mouse XRCC6

GeneID: 2547 Human XRCC6

Swiss-port # P12956 Human X-ray repair cross-complementing protein 6

Swiss-port # P23475 Mouse X-ray repair cross-complementing protein 6

Gene Symbol XRCC6
Gene Full Name X-ray repair complementing defective repair in Chinese hamster cells 6
Background The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]
Function Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. [UniProt]
Research Area Cancer antibody; Gene Regulation antibody
Calculated MW 70 kDa
PTM Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.

Clone References

Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks.

WB / Human

Cheng Q et al.
Nucleic Acids Res.,  (2011)

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Interplay between Ku, Artemis, and the DNA-dependent protein kinase catalytic subunit at DNA ends.

IP / Human

Drouet J et al.
J Biol Chem.,  (2006)

publication_link

 

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Identification and functional analysis of a human homologue of the monkey replication origin ors8.

ChIP / Human, Primates

Callejo M et al.
J Cell Biochem.,  (2006)

publication_link

 

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Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.

GSA / Human

Sawchuk DJ et al.
J Biol Chem.,  (2004)

publication_link

 

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Analysis of the DNA replication competence of the xrs-5 mutant cells defective in Ku86.

FACS / Hamster

Matheos D et al.
J Cell Sci.,  (2003)

publication_link

 

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In vivo association of Ku with mammalian origins of DNA replication.

IP / Primates

Novac O et al.
Mol Biol Cell.,  (2001)

publication_link

 

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Elevated breast cancer risk in irradiated BALB/c mice associates with unique functional polymorphism of the Prkdc (DNA-dependent protein kinase catalytic subunit) gene.

WB / Mouse, Pig

Yu Y et al.
Cancer Res.,  (2001)

publication_link

 

hr_line

Hypoxia-activated ligand HAL-1/13 is lupus autoantigen Ku80 and mediates lymphoid cell adhesion in vitro.

IHC / Human

Lynch EM et al.
Am J Physiol Cell Physiol.,  (2001)

publication_link

 

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Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens.

ICC/IF, IHC-P, Puri, FACS / Human, Monkey, Mouse, Xenopus laevis, Rat

Wang J et al.
Mol Biol Rep.,  (1993)

publication_link

 

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