ARG64246

anti-Ku 70 antibody

anti-Ku 70 antibody for Western blot and Human

Cancer antibody; Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes Ku 70
Tested Reactivity Hu
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Ku 70
Antigen Species Human
Immunogen YKTEGDEEAEEEQ-C
Conjugation Un-conjugated
Alternate Names DNA repair protein XRCC6; Thyroid-lupus autoantigen; Lupus Ku autoantigen protein p70; EC 4.2.99.-; EC 3.6.4.-; ATP-dependent DNA helicase II 70 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 6; CTC box-binding factor 75 kDa subunit; 70 kDa subunit of Ku antigen; CTC75; 5'-deoxyribose-5-phosphate lyase Ku70; KU70; TLAA; 5'-dRP lyase Ku70; CTCBF; ML8; G22P1; X-ray repair cross-complementing protein 6; ATP-dependent DNA helicase 2 subunit 1; Ku70

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2547 Human XRCC6

Swiss-port # P12956 Human X-ray repair cross-complementing protein 6

Background The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]
Research Area Cancer antibody; Gene Regulation antibody
Calculated MW 70 kDa
PTM Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.