ARG62529
anti-Ku 70 antibody [N3H10]
anti-Ku 70 antibody [N3H10] for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot,Flow cytometry,ICC/IF and Mouse,Human,Monkey
Cancer antibody; Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [N3H10] recognizes Ku 70 |
|---|---|
| Tested Reactivity | Hu, Ms, Mk |
| Tested Application | FACS, ICC/IF, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | N3H10 |
| Isotype | IgG2b |
| Target Name | Ku 70 |
| Antigen Species | Human |
| Immunogen | Human placental extract designated as PSE1-PL. |
| Epitope | Amino acids 506 - 541 |
| Conjugation | Un-conjugated |
| Alternate Names | DNA repair protein XRCC6; Thyroid-lupus autoantigen; Lupus Ku autoantigen protein p70; EC 4.2.99.-; EC 3.6.4.-; ATP-dependent DNA helicase II 70 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 6; CTC box-binding factor 75 kDa subunit; 70 kDa subunit of Ku antigen; CTC75; 5'-deoxyribose-5-phosphate lyase Ku70; KU70; TLAA; 5'-dRP lyase Ku70; CTCBF; ML8; G22P1; X-ray repair cross-complementing protein 6; ATP-dependent DNA helicase 2 subunit 1; Ku70 |
Application Instructions
| Application Note | WB: 0.25 - 0.5 µg/ml IP: 2µg/mg of protein lysate IF: 1 µg/ml FACS: 1µg for 106 cells IHC: 1 - 2 µg/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P12956 Human X-ray repair cross-complementing protein 6 Swiss-port # P23475 Mouse X-ray repair cross-complementing protein 6 |
|---|---|
| Gene Symbol | XRCC6 |
| Gene Full Name | X-ray repair complementing defective repair in Chinese hamster cells 6 |
| Background | The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008] |
| Function | Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. [UniProt] |
| Cellular Localization | Nucleus |
| Research Area | Cancer antibody; Gene Regulation antibody |
| Calculated MW | 70 kDa |
| PTM | Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair. |
Clone References
ATR pathway inhibition is synthetically lethal in cancer cells with ERCC1 deficiency.
P21-PARP-1 pathway is involved in cigarette smoke-induced lung DNA damage and cellular senescence.
WB / Mouse
A mechanism for the inhibition of DNA-PK-mediated DNA sensing by a virus.
WB / Human
A noncatalytic function of the ligation complex during nonhomologous end joining.
WB / Human
Telomere dysfunction-induced foci arise with the onset of telomeric deletions and complex chromosomal aberrations in resistant chronic lymphocytic leukemia cells.
ChIP / Human
Rad54B targeting to DNA double-strand break repair sites requires complex formation with S100A11.
ICC/IF / Human
Treatment of PC12 cells with nerve growth factor induces proteasomal degradation of T-cadherin that requires tyrosine phosphorylation of its cadherin domain.
WB / Rat
Expression and chromosome location of hamster Ku70 and Ku80.
WB / Hamster
A method to detect particle-specific antibodies against Ku and the DNA-dependent protein kinase catalytic subunit in autoimmune sera.
IP / Human
Hypoxia-activated ligand HAL-1/13 is lupus autoantigen Ku80 and mediates lymphoid cell adhesion in vitro.
FACS / Human
