ARG62529

anti-Ku 70 antibody [N3H10]

anti-Ku 70 antibody [N3H10] for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot,Flow cytometry,ICC/IF and Mouse,Human,Monkey

Cancer antibody; Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody [N3H10] recognizes Ku 70
Tested Reactivity Hu, Ms, Mk
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Clone N3H10
Isotype IgG2b
Target Name Ku 70
Antigen Species Human
Immunogen Human placental extract designated as PSE1-PL.
Epitope Amino acids 506 - 541
Conjugation Un-conjugated
Alternate Names DNA repair protein XRCC6; Thyroid-lupus autoantigen; Lupus Ku autoantigen protein p70; EC 4.2.99.-; EC 3.6.4.-; ATP-dependent DNA helicase II 70 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 6; CTC box-binding factor 75 kDa subunit; 70 kDa subunit of Ku antigen; CTC75; 5'-deoxyribose-5-phosphate lyase Ku70; KU70; TLAA; 5'-dRP lyase Ku70; CTCBF; ML8; G22P1; X-ray repair cross-complementing protein 6; ATP-dependent DNA helicase 2 subunit 1; Ku70

Application Instructions

Application Note WB: 0.25 - 0.5 µg/ml
IP: 2µg/mg of protein lysate
IF: 1 µg/ml
FACS: 1µg for 106 cells
IHC: 1 - 2 µg/ml
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A purified
Buffer 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Stabilizer 0.2% BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14375 Mouse XRCC6

GeneID: 2547 Human XRCC6

Swiss-port # P12956 Human X-ray repair cross-complementing protein 6

Swiss-port # P23475 Mouse X-ray repair cross-complementing protein 6

Gene Symbol XRCC6
Gene Full Name X-ray repair complementing defective repair in Chinese hamster cells 6
Background The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]
Function Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. [UniProt]
Cellular Localization Nucleus
Research Area Cancer antibody; Gene Regulation antibody
Calculated MW 70 kDa
PTM Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.

Clone References

ATR pathway inhibition is synthetically lethal in cancer cells with ERCC1 deficiency.

Mohni KN et al.
Cancer Res.,  (2014)

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P21-PARP-1 pathway is involved in cigarette smoke-induced lung DNA damage and cellular senescence.

WB / Mouse

Yao H et al.
PLoS One.,  (2013)

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hr_line

A mechanism for the inhibition of DNA-PK-mediated DNA sensing by a virus.

WB / Human

Peters NE et al.
PLoS Pathog.,  (2013)

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A noncatalytic function of the ligation complex during nonhomologous end joining.

WB / Human

Cottarel J et al.
J Cell Biol.,  (2013)

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Telomere dysfunction-induced foci arise with the onset of telomeric deletions and complex chromosomal aberrations in resistant chronic lymphocytic leukemia cells.

ChIP / Human

Brugat T et al.
Blood.,  (2010)

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Rad54B targeting to DNA double-strand break repair sites requires complex formation with S100A11.

ICC/IF / Human

Murzik U et al.
Mol Biol Cell.,  (2008)

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Treatment of PC12 cells with nerve growth factor induces proteasomal degradation of T-cadherin that requires tyrosine phosphorylation of its cadherin domain.

WB / Rat

Bai S et al.
J Biol Chem.,  (2007)

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hr_line

Expression and chromosome location of hamster Ku70 and Ku80.

WB / Hamster

Koike M et al.
Cytogenet Cell Genet.,  (2001)

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A method to detect particle-specific antibodies against Ku and the DNA-dependent protein kinase catalytic subunit in autoimmune sera.

IP / Human

Jafri F et al.
J Immunol Methods.,  (2001)

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Hypoxia-activated ligand HAL-1/13 is lupus autoantigen Ku80 and mediates lymphoid cell adhesion in vitro.

FACS / Human

Lynch EM et al.
Am J Physiol Cell Physiol.,  (2001)

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