ARG57582
anti-LAPTM4B antibody
anti-LAPTM4B antibody for Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes LAPTM4B |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | LAPTM4B |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 195-209 of Human LAPTM4B. |
| Conjugation | Un-conjugated |
| Alternate Names | Lysosome-associated transmembrane protein 4-beta; LAPTM4beta; LC27; Lysosomal-associated transmembrane protein 4B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q5U1W4 Rat Lysosomal-associated transmembrane protein 4B Swiss-port # Q86VI4 Human Lysosomal-associated transmembrane protein 4B |
|---|---|
| Gene Symbol | LAPTM4B |
| Gene Full Name | lysosomal protein transmembrane 4 beta |
| Function | Required for optimal lysosomal function (PubMed:21224396). Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed:25588945). Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed:25998567). Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed:26126825). Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed:26280656). [UniProt] |
| Cellular Localization | Endomembrane system; Multi-pass membrane protein |
| Calculated MW | 35 kDa |
