ARG65221
anti-LARGE1 antibody
anti-LARGE1 antibody for Western blot and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes LARGE1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow, Dog |
| Tested Application | WB |
| Specificity | Reported variants represent identical protein: NP_004728.1, NP_598397.1 |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | LARGE1 |
| Antigen Species | Human |
| Immunogen | C-SEADVNSENLQKQ |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.4.1.-; MDC1D; EC 2.4.-.-; Acetylglucosaminyltransferase-like 1A; MDDGA6; MDDGB6; Glycosyltransferase-like protein LARGE1; EC 2.4.2.- |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O95461 Human Glycosyltransferase-like protein LARGE1 |
|---|---|
| Gene Symbol | LARGE1 |
| Gene Full Name | LARGE xylosyl- and glucuronyltransferase 1 |
| Background | This gene, which is one of the largest in the human genome, encodes a member of the N-acetylglucosaminyltransferase gene family. It encodes a glycosyltransferase which participates in glycosylation of alpha-dystroglycan, and may carry out the synthesis of glycoprotein and glycosphingolipid sugar chains. It may also be involved in the addition of a repeated disaccharide unit. Mutations in this gene cause MDC1D, a novel form of congenital muscular dystrophy with severe mental retardation and abnormal glycosylation of alpha-dystroglycan. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008] |
| Function | Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:22223806). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration. LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (PubMed:25279699). [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 88 kDa |
