ARG42380
anti-LARGE1 antibody [LARGE-02] (PE)
anti-LARGE1 antibody [LARGE-02] (PE) for Flow cytometry and Human
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [LARGE-02] recognizes LARGE1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The mouse monoclonal antibody LARGE-02 recognizes human LARGE1, a glycosyltransferase expressed mainly in the Golgi apparatus. Crossreactivity with LARGE2 was not determined. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | LARGE-02 |
| Isotype | IgG2b |
| Target Name | LARGE1 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment corresponding to aa. 35-142 of Human LARGE1. |
| Conjugation | PE |
| Alternate Names | EC 2.4.1.-; MDC1D; EC 2.4.-.-; Acetylglucosaminyltransferase-like 1A; MDDGA6; MDDGB6; Glycosyltransferase-like protein LARGE1; EC 2.4.2.- |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O95461 Human Glycosyltransferase-like protein LARGE1 |
|---|---|
| Gene Symbol | LARGE |
| Gene Full Name | like-glycosyltransferase |
| Background | This gene encodes a member of the N-acetylglucosaminyltransferase gene family. It encodes a glycosyltransferase which participates in glycosylation of alpha-dystroglycan, and may carry out the synthesis of glycoprotein and glycosphingolipid sugar chains. It may also be involved in the addition of a repeated disaccharide unit. The protein encoded by this gene is the glycotransferase that adds the final xylose and glucuronic acid to alpha-dystroglycan and thereby allows alpha-dystroglycan to bind ligands including laminin 211 and neurexin. Mutations in this gene cause several forms of congenital muscular dystrophy characterized by cognitive disability and abnormal glycosylation of alpha-dystroglycan. Alternative splicing of this gene results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2018] |
| Function | Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:22223806). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration. LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (PubMed:25279699). [UniProt] |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein. [UniProt] |
| Calculated MW | 88 kDa |
