ARG51300

anti-LC3A antibody

anti-LC3A antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes LC3A

Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name LC3A
Antigen Species Human
Immunogen Peptide sequence around aa.5~10 (R-P-F-K-Q) derived from Human LC3A.
Conjugation Un-conjugated
Alternate Names MAP1A/MAP1B light chain 3 A; MAP1BLC3; LC3; MAP1 light chain 3-like protein 1; MAP1A/MAP1B LC3 A; Autophagy-related protein LC3 A; Microtubule-associated protein 1 light chain 3 alpha; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3A; LC3A; ATG8E; Autophagy-related ubiquitin-like modifier LC3 A

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-PAssay-dependent
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 362245 Rat MAP1LC3A

GeneID: 66734 Mouse MAP1LC3A

GeneID: 84557 Human MAP1LC3A

Gene Symbol MAP1LC3A
Gene Full Name microtubule-associated protein 1 light chain 3 alpha
Background Probably involved in formation of autophagosomal vacuoles (autophagosomes).
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30273 LC3A and LC3B Antibody Duo
Related products:
LC3A antibodies; LC3A Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 14 kDa
PTM The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094).
The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.

Customer's Feedback

nuts_pic      Excellent

Review for anti-LC3A antibody

Application:WB

Sample:HeLa or HeLa CQ 50 µM

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-LC3A antibody

Application:WB

Sample:HeLa untreated or treated with Chloroquine at 50 uM, 100 uM (18hr)

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-LC3A antibody

Application:WB

Sample:Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-LC3A antibody

Application:WB

Sample:Mouse brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Role of Autophagy in Acquired Cholesteatoma.

WB, IHC-P / Human

Li Quancheng et al.
Otol Neurotol.,  (2019)

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