ARG43536

anti-LC3B antibody

anti-LC3B antibody for Western blot,ICC/IF,Immunoprecipitation and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes LC3B.
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name LC3B
Antigen Species Human
Immunogen Synthetic peptide derived from human LC3B
Protein Full Name Microtubule-associated proteins 1A/1B light chain 3B
Alternate Names LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IP1:20 - 1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 64862 Rat MAP1LC3B

GeneID: 67443 Mouse MAP1LC3B

GeneID: 81631 Human MAP1LC3B

Gene Symbol MAP1LC3B
Gene Full Name microtubule-associated protein 1 light chain 3 beta
Background The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. [UniProt]