ARG54708

anti-LC3C antibody

anti-LC3C antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes LC3C
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name LC3C
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 1-30 (N-terminus) of Human LC3C (NP_001004343.1).
Conjugation Un-conjugated
Alternate Names Microtubule-associated proteins 1A/1B light chain 3C; MAP1A/MAP1B light chain 3 C; ATG8J; Microtubule-associated protein 1 light chain 3 gamma; MAP1 light chain 3-like protein 3; MAP1A/MAP1B LC3 C; Autophagy-related protein LC3 C; LC3C; Autophagy-related ubiquitin-like modifier LC3 C

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control T47D

Properties

Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 440738 Human MAP1LC3C

Swiss-port # Q9BXW4 Human Microtubule-associated proteins 1A/1B light chain 3C

Gene Symbol MAP1LC3C
Gene Full Name microtubule-associated protein 1 light chain 3 gamma
Background Autophagy is a highly regulated bulk degradation process that plays an important role in cellular maintenance and development. MAP1LC3C is an ortholog of the yeast autophagosome protein Atg8 (He et al., 2003 [PubMed 12740394]).[supplied by OMIM, Nov 2010]
Function Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2. Recruites all ATG8 family members to infecting bacteria such as S.Typhimurium. [From Uniprot]
Cellular Localization Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 17 kDa
PTM The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094).
The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.