ARG54708
anti-LC3C antibody
anti-LC3C antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Overview
Product Description | Rabbit Polyclonal antibody recognizes LC3C |
---|---|
Tested Reactivity | Hu |
Tested Application | IHC-P, WB |
Host | Rabbit |
Clonality | Polyclonal |
Target Name | LC3C |
Antigen Species | Human |
Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 1-30 (N-terminus) of Human LC3C (NP_001004343.1). |
Conjugation | Un-conjugated |
Alternate Names | Microtubule-associated proteins 1A/1B light chain 3C; MAP1A/MAP1B light chain 3 C; ATG8J; Microtubule-associated protein 1 light chain 3 gamma; MAP1 light chain 3-like protein 3; MAP1A/MAP1B LC3 C; Autophagy-related protein LC3 C; LC3C; Autophagy-related ubiquitin-like modifier LC3 C |
Application Instructions
Application Suggestion |
|
||||||
---|---|---|---|---|---|---|---|
Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
Positive Control | T47D |
Properties
Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
---|---|
Buffer | PBS and 0.09% (W/V) Sodium azide |
Preservative | 0.09% (W/V) Sodium azide |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # Q9BXW4 Human Microtubule-associated proteins 1A/1B light chain 3C |
---|---|
Gene Symbol | MAP1LC3C |
Gene Full Name | microtubule-associated protein 1 light chain 3 gamma |
Background | Autophagy is a highly regulated bulk degradation process that plays an important role in cellular maintenance and development. MAP1LC3C is an ortholog of the yeast autophagosome protein Atg8 (He et al., 2003 [PubMed 12740394]).[supplied by OMIM, Nov 2010] |
Function | Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2. Recruites all ATG8 family members to infecting bacteria such as S.Typhimurium. [From Uniprot] |
Cellular Localization | Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes |
Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
Calculated MW | 17 kDa |
PTM | The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094). The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. |