ARG63060

anti-LIME antibody [LIME-10]

anti-LIME antibody [LIME-10] for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [LIME-10] recognizes LIME
Tested Reactivity Hu
Tested Application IHC-P, WB
Specificity The clone LIME-10 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
Host Mouse
Clonality Monoclonal
Clone LIME-10
Isotype IgG2a
Target Name LIME
Antigen Species Human
Immunogen COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin.
Conjugation Un-conjugated
Alternate Names Lck-interacting molecule; Lck-interacting membrane protein; Lck-interacting transmembrane adapter 1; dJ583P15.4; LIME

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P10 µg/ml
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 54923 Human LIME1

Swiss-port # Q9H400 Human Lck-interacting transmembrane adapter 1

Gene Symbol LIME1
Gene Full Name Lck interacting transmembrane adaptor 1
Background LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Function Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules. [UniProt]
Research Area Immune System antibody
Calculated MW 31 kDa
PTM Palmitoylation of Cys-28 and Cys-31 is required for raft targeting.
Phosphorylated on tyrosines upon TCR activation and/or CD4 coreceptor stimulation, or upon BCR stimulation; which leads to the recruitment of SH2-containing proteins.

Clone References

LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling.

Brdicková N et al.
J Exp Med.,  (2003)

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