ARG63063

anti-LIME antibody [mLIME-05]

anti-LIME antibody [mLIME-05] for Immunoprecipitation,Western blot and Mouse

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [mLIME-05] recognizes LIME
Tested Reactivity Ms
Tested Application IP, WB
Specificity The clone mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells._x000D_
Host Mouse
Clonality Monoclonal
Clone mLIME-05
Isotype IgG2a
Target Name LIME
Antigen Species Mouse
Immunogen Recombinant protein corresponding to amino acids 86-202 of mouse LIME_x000D_
Conjugation Un-conjugated
Alternate Names Lck-interacting molecule; Lck-interacting membrane protein; Lck-interacting transmembrane adapter 1; dJ583P15.4; LIME

Application Instructions

Application Suggestion
Tested Application Dilution
IPAssay-dependent
WB1 - 5 µg/ml
Application Note WB: Under reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IP: Murine splenic T cells

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 72699 Mouse LIME1

Swiss-port # Q9EQR5 Mouse Lck-interacting transmembrane adapter 1

Gene Symbol Lime1
Gene Full Name Lck interacting transmembrane adaptor 1
Background LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals. _x000D_
Function Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules. [UniProt]
Research Area Immune System antibody
Calculated MW 31 kDa
PTM Palmitoylation of Cys-28 and Cys-31 is required for raft targeting.
Phosphorylated on tyrosines upon TCR activation and/or CD4 coreceptor stimulation, or upon BCR stimulation; which leads to the recruitment of SH2-containing proteins.