ARG56576

anti-LIPE / HSL antibody

anti-LIPE / HSL antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes LIPE / HSL
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Specificity Multiple isoforms of LIPE / HSL are known to exist.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name LIPE / HSL
Immunogen Synthetic peptide (14 aa) within aa. 920-970 of Human LIPE / HSL.
Conjugation Un-conjugated
Alternate Names EC 3.1.1.79; AOMS4; HSL; LHS; Hormone-sensitive lipase; FPLD6

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF20 µg/ml
IHC-P2.5 µg/ml
WB0.5 - 1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human lymph node tissue.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16890 Mouse LIPE

GeneID: 25330 Rat LIPE

GeneID: 3991 Human LIPE

Gene Symbol LIPE
Gene Full Name lipase, hormone-sensitive
Background The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008]
Function In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. [UniProt]
Calculated MW Isoform 1: 117 kDa
Isoform 2: 84 kDa
PTM Phosphorylation by AMPK may block translocation to lipid droplets.