ARG63216

anti-LIS1 / PAFAH1B1 antibody

anti-LIS1 / PAFAH1B1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes LIS1 / PAFAH1B1
Tested Reactivity Hu, Rat
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name LIS1 / PAFAH1B1
Antigen Species Human
Immunogen TGSVDQTVKVWECR
Conjugation Un-conjugated
Alternate Names Platelet-activating factor acetylhydrolase IB subunit alpha; LIS-1; MDCR; LIS1; LIS2; PAF acetylhydrolase 45 kDa subunit; PAF-AH 45 kDa subunit; PAF-AH alpha; Lissencephaly-1 protein; PAFAH; MDS; PAFAH alpha

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5048 Human PAFAH1B1

GeneID: 83572 Rat PAFAH1B1

Swiss-port # P43034 Human Platelet-activating factor acetylhydrolase IB subunit alpha

Swiss-port # P63004 Rat Platelet-activating factor acetylhydrolase IB subunit alpha

Background This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum. [provided by RefSeq, Apr 2009]
Research Area Neuroscience antibody
Calculated MW 47 kDa