ARG23654
anti-LPL / Lipoprotein Lipase antibody [5D2] (low endotoxin)
anti-LPL / Lipoprotein Lipase antibody [5D2] (low endotoxin) for ELISA,Functional study,Immunoprecipitation,Western blot and Human,Rat,Bovine,Chicken,Guinea pig
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [5D2] recognizes LPL / Lipoprotein Lipase |
|---|---|
| Tested Reactivity | Hu, Rat, Bov, Chk, Gpig |
| Species Does Not React With | Ms |
| Tested Application | ELISA, FuncSt, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5D2 |
| Isotype | IgG1 |
| Target Name | LPL / Lipoprotein Lipase |
| Antigen Species | Bovine |
| Immunogen | Purified Bovine milk lipoprotein lipase. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 3.1.1.34; LPL; Lipoprotein lipase; LIPD; HDLCQ11 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: This product detects a band of ~ 53 kDa using partially purified LPL from postheparin plasma. ELISA: This product can be used in an indirect ELISA, or as the capture antibody in a sandwich ELISA. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Purification Note | Low endotoxin |
| Buffer | PBS. |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LPL |
| Gene Full Name | lipoprotein lipase |
| Background | LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008] |
| Function | The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium (By similarity). [UniProt] |
| Calculated MW | 53 kDa |
| PTM | Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity. [UniProt] |
