ARG58184

anti-LRP1 antibody

anti-LRP1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes LRP1
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name LRP1
Antigen Species Human
Immunogen Synthetic peptide derived from Human LRP1.
Conjugation Un-conjugated
Alternate Names Apolipoprotein E receptor; APOER; Alpha-2-macroglobulin receptor; LRP-1; TGFBR5; LRP1A; APR; Prolow-density lipoprotein receptor-related protein 1; A2MR; CD antigen CD91; CD91; IGFBP3R; LRP; LRP-85; LRPICD; LRP-515

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:50 - 1:100
IHC-P1:50 - 1:100
IP1:30
WB1:10000 - 1:20000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16971 Mouse LRP1

GeneID: 4035 Human LRP1

Swiss-port # Q07954 Human Prolow-density lipoprotein receptor-related protein 1

Swiss-port # Q91ZX7 Mouse Prolow-density lipoprotein receptor-related protein 1

Gene Symbol LRP1
Gene Full Name low density lipoprotein receptor-related protein 1
Background The protein encoded by this gene is an endocytic receptor involved in several cellular processes, including intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. In addition, the encoded protein is necessary for the A2M-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients. Expression of this gene decreases with age and has been found to be lower than controls in brain tissue from Alzheimer patients. [provided by RefSeq, Jan 2010]
Function Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.

Functions as a receptor for Pseudomonas aeruginosa exotoxin A. [UniProt]
Cellular Localization Cell surface. [UniProt]
Calculated MW 505 kDa
PTM Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.

The N-terminus is blocked.

Phosphorylated on serine and threonine residues.

Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1. [UniProt]