ARG65177

anti-LSP1 antibody

anti-LSP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Immune System antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes LSP1
Tested Reactivity Hu, Ms
Predict Reactivity Cow, Rat, Dog
Tested Application IHC-P, WB
Specificity This antibody is expected to recognize both reported isoforms (NP_002330.1; NP_001013271.1). Reported variants represent identical protein: NP_001013273.1, NP_001013271.1, NP_001013272.1
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name LSP1
Antigen Species Human
Immunogen C-RYKFVATGHGKYEKV
Conjugation Un-conjugated
Alternate Names Lymphocyte-specific protein 1; pp52; 52 kDa phosphoprotein; 47 kDa actin-binding protein; WP34; Lymphocyte-specific antigen WP34

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB0.01 - 0.1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16985 Mouse LSP1

GeneID: 4046 Human LSP1

Swiss-port # P19973 Mouse Lymphocyte-specific protein 1

Swiss-port # P33241 Human Lymphocyte-specific protein 1

Background This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Research Area Immune System antibody; Signaling Transduction antibody
Calculated MW 37 kDa
PTM Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis (By similarity).