ARG65475
anti-LST1 antibody [LST1/02]
anti-LST1 antibody [LST1/02] for ICC/IF,Immunoprecipitation,Western blot and Human
Cell Biology and Cellular Response antibody; Immune System antibody; Neuroscience antibody
Overview
| Product Description | Mouse Monoclonal antibody [LST1/02] recognizes LST1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IP, WB |
| Specificity | The clone LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11 kDa protein expressed as various transmembrane or soluble forms. LST1 is found predominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels as approximately 25-28 kDa molecule._x000D_ |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | LST1/02 |
| Isotype | IgG1 |
| Target Name | LST1 |
| Antigen Species | Human |
| Immunogen | KLH-fused peptide corresponding to amino acids SSEGPDLRGRDKRGT of human LST1_x000D_ |
| Conjugation | Un-conjugated |
| Alternate Names | Protein B144; B144; D6S49E; LST-1; Leukocyte-specific transcript 1 protein |
Application Instructions
| Application Suggestion |
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| Application Note | WB: It migrates on SDS-PAGE gels as ~ 25-28 kDa molecule. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | IP: Raji |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O00453 Human Leukocyte-specific transcript 1 protein |
|---|---|
| Gene Symbol | LST1 |
| Gene Full Name | leukocyte specific transcript 1 |
| Background | LST1 (leukocyte-specific transcript, also known as B144) is expressed in cells of myeloid/erythroid lineage (monocytes, granulocytes, dendritic cells, platelets, erythrocytes). At least 14 alternatively spliced variants (LST1/A – LST1/N) can be detected; some of them (LST1/A, B, C, G, I, K) are transmembrane cell surface-exposed forms, the other are soluble. LST1 induces production of long, thin filopodia in dendritic cells, has an inhibitory effect on lymphocyte proliferation and may have an immunomodulatory role. LST1/A is an 11 kDa transmembrane adaptor present in membrane rafts and forms spontaneously covalent homodimers. Its intracellular domain contains two tyrosine motifs, one of them being an ITIM very similar to such motifs in Siglec._x000D_ |
| Function | Possible role in modulating immune responses. Induces morphological changes including production of filopodia and microspikes when overexpressed in a variety of cell types and may be involved in dendritic cell maturation. Isoform 1 and isoform 2 have an inhibitory effect on lymphocyte proliferation. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Immune System antibody; Neuroscience antibody |
| Calculated MW | 11 kDa |
