ARG55125

anti-Lactate Dehydrogenase B antibody

anti-Lactate Dehydrogenase B antibody for Immunohistochemistry,Western blot and Human,Mouse,Rat

Cancer antibody; Metabolism antibody; Signaling Transduction antibody; Lactate dehydrogenase antibody; Warburg Effect signaling pathway antibody
nuts_pic
1

Overview

Product Description

Rabbit Polyclonal antibody recognizes Lactate Dehydrogenase B

Tested Reactivity Hu, Ms, Rat
Tested Application IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Lactate Dehydrogenase B
Antigen Species Human
Immunogen Recombinant protein of Human LDHB (Swiss: P07195)
Conjugation Un-conjugated
Alternate Names Renal carcinoma antigen NY-REN-46; LDH heart subunit; TRG-5; LDHBD; LDH-H; EC 1.1.1.27; LDH-B; HEL-S-281; L-lactate dehydrogenase B chain

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16832 Mouse LDHB

GeneID: 24534 Rat LDHB

GeneID: 3945 Human LDHB

Gene Symbol LDHB
Gene Full Name lactate dehydrogenase B
Background This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2010]
Function Catalytic activity: (S)-lactate + NAD+ = pyruvate + NADH. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30261 Lactate dehydrogenase Antibody Duo (LDHA,LDHB)
Related products:
Lactate Dehydrogenase antibodies; Lactate Dehydrogenase ELISA Kits; Lactate Dehydrogenase Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
A non-autophatic role of Atg9a in necrosis and developmental bone formation
Research Area Cancer antibody; Metabolism antibody; Signaling Transduction antibody; Lactate dehydrogenase antibody; Warburg Effect signaling pathway antibody
Calculated MW 37 kDa

Customer's Feedback

nuts_pic      Excellent

Review for anti-Lactate Dehydrogenase B antibody

Application:WB

Sample:Rat heart

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC