ARG40694

anti-Lamin B1 antibody

anti-Lamin B1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunocytochemistry and Human,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Signaling Transduction antibody; NRF-2 Nuclear Localization Study antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Lamin B1
Tested Reactivity Hu, Rat
Predict Reactivity Hm
Tested Application ICC, IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Lamin B1
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 570-586 of Human Lamin B1. (FHQQGTPRASNRSCAIM)
Conjugation Un-conjugated
Alternate Names LMN2; ADLD; Lamin-B1; LMN; LMNB

Application Instructions

Application Suggestion
Tested Application Dilution
ICC1:200 - 1:1000
IHC-Fr1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Thimerosal and 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 116685 Rat LMNB1

GeneID: 4001 Human LMNB1

Swiss-port # P20700 Human Lamin-B1

Swiss-port # P70615 Rat Lamin-B1

Gene Symbol LMNB1
Gene Full Name lamin B1
Background The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010]
Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. [UniProt]
Cellular Localization Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Signaling Transduction antibody; NRF-2 Nuclear Localization Study antibody
Calculated MW 66 kDa
PTM B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. [UniProt]