ARG65654

anti-Lamin B1 antibody

anti-Lamin B1 antibody for Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Signaling Transduction antibody; NRF-2 Nuclear Localization Study antibody

Overview

Product Description Goat Polyclonal antibody recognizes Lamin B1
Tested Reactivity Hu, Ms
Predict Reactivity Cow, Dog, Pig
Tested Application WB
Specificity This antibody is expected to recognize both reported isoforms (NP_005564.1; NP_001185486.1).
Host Goat
Clonality Polyclonal
Target Name Lamin B1
Antigen Species Human
Immunogen Synthetic peptide around the internal region of Human Lamin B1 (C-DVKVILKNSQGE)
Conjugation Un-conjugated
Alternate Names LMN2; ADLD; Lamin-B1; LMN; LMNB

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16906 Mouse LMNB1

GeneID: 4001 Human LMNB1

Swiss-port # P14733 Mouse Lamin-B1

Swiss-port # P20700 Human Lamin-B1

Gene Symbol LMNB1
Gene Full Name lamin B1
Background The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010]
Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Signaling Transduction antibody; NRF-2 Nuclear Localization Study antibody
Calculated MW 66.4 kDa (NP_005564.1)
PTM B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.