ARG22484
anti-Laminin alpha 2 antibody [5H2]
anti-Laminin alpha 2 antibody [5H2] for ELISA,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Monkey,Rabbit
Overview
| Product Description | Mouse Monoclonal antibody [5H2] recognizes Laminin alpha 2 This antibody recognizes the ~80 kDa fragment of laminin subunit alpha-2, also known as the laminin M chain, laminin-12 subunit alpha, laminin-2 subunit alpha, laminin-4 subunit alpha or merosin heavy chain. Laminins are trimeric basement glycoproteins consisting of three non-identical multi-domain chains (alpha, beta and gamma) each encoded by a distinct gene. The alpha-2 chain is a subunit of Laminin-2 and Laminin-4, widely expressed in the basement membrane of skeletal muscle and peripheral nerves. Laminins are thought to mediate cell attachment, migration, proliferation and differentiation with other extracellular matrix components.Mutation of the LAMA2 gene can lead to the development of Merosin-deficient congenital muscular dystrophy 1A (MDC1A), a condition characterized by hypotonia, proximal weakness, hyporeflexia and difficulty walking (Tazek et al. 2003). |
|---|---|
| Tested Reactivity | Hu, Mk, Rb |
| Tested Application | ELISA, ICC/IF, IHC-Fr, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5H2 |
| Isotype | IgG1 |
| Target Name | Laminin alpha 2 |
| Antigen Species | Human |
| Immunogen | Purified Human Merosin. |
| Conjugation | Un-conjugated |
| Alternate Names | Laminin-12 subunit alpha; Laminin-2 subunit alpha; Laminin-4 subunit alpha; Merosin heavy chain; Laminin M chain; LAMM; Laminin subunit alpha-2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-Fr: Acetone is recommended. 8 µm fixed cryostat muscle sections have been used, with an HRP conjugated secondary antibody for detection. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | Ascites and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | LAMA2 |
| Gene Full Name | laminin, alpha 2 |
| Background | Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. [UniProt] |
| Calculated MW | 344 kDa |
