ARG63058

anti-Lck antibody [LCK-01]

anti-Lck antibody [LCK-01] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot,CyTOF®-candidate and Human

Immune System antibody; Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody

Overview

Product Description

Mouse Monoclonal antibody [LCK-01] recognizes Lck

Tested Reactivity Hu
Species Does Not React With Ms
Tested Application CyTOF®-candidate, FACS, ICC/IF, IP, WB
Specificity The clone LCK-01 recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase.
Host Mouse
Clonality Monoclonal
Clone LCK-01
Isotype IgG1
Target Name Lck
Antigen Species Human
Immunogen Peptide corresponding to amino acids 22-36 in the sequence of human Lck.
Conjugation Un-conjugated
Alternate Names T cell-specific protein-tyrosine kinase; Leukocyte C-terminal Src kinase; Protein YT16; p56-LCK; LSK; Proto-oncogene Lck; p56lck; pp58lck; Tyrosine-protein kinase Lck; YT16; IMD22; Lymphocyte cell-specific protein-tyrosine kinase; EC 2.7.10.2

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 5 µg/ml
ICC/IFAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3932 Human LCK

Swiss-port # P06239 Human Tyrosine-protein kinase Lck

Gene Symbol LCK
Gene Full Name LCK proto-oncogene, Src family tyrosine kinase
Background Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its zeta subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors.
Function Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30169 Src Family Protein Tyrosine Kinases Antibody Panel
Related products:
Lck antibodies; Lck Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Immune System antibody; Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody
Calculated MW 58 kDa
PTM Autophosphorylated on Tyr-394, increasing enzymatic activity, this site is dephosphorylated by PTN22. Phosphorylated on Tyr-505 by CSK, decreasing activity. Dephosphorylated by PTPRC/CD45. Dephosphorylation at Tyr-394 by PTPN2 negatively regulates T-cell receptor signaling.
Myristoylation is required prior to palmitoylation.
Palmitoylation regulates subcellular location.

Clone References

A potential role for protein tyrosine kinase p56(lck) in rheumatoid arthritis synovial fluid T lymphocyte hyporesponsiveness.

Romagnoli P et al.
Int Immunol.,  (2001)

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Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells.

Cebecauer M et al.
Biochem Biophys Res Commun.,  (1998)

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