ARG40136

anti-Lunatic Fringe antibody

anti-Lunatic Fringe antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Lunatic Fringe
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Lunatic Fringe
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 86-114 of Human Lunatic Fringe.
Conjugation Un-conjugated
Alternate Names O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; EC 2.4.1.222; SCDO3; Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-P1:50 - 1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3955 Human LFNG

Swiss-port # Q8NES3 Human Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe

Gene Symbol LFNG
Gene Full Name LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Background This gene is a member of the fringe gene family which also includes radical and manic fringe genes. They all encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This gene product is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Function Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning (By similarity). [UniProt]
Cellular Localization Golgi apparatus membrane; Single-pass type II membrane protein. [UniProt]
Calculated MW 42 kDa
PTM A soluble form may be derived from the membrane form by proteolytic processing. [UniProt]