ARG59962
anti-Lunatic Fringe antibody
anti-Lunatic Fringe antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Lunatic Fringe |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Lunatic Fringe |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-250 of Human Lunatic Fringe (NP_002295.1). |
| Conjugation | Un-conjugated |
| Alternate Names | O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; EC 2.4.1.222; SCDO3; Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse spleen and HeLa | ||||||
| Observed Size | 42 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O09010 Mouse Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe Swiss-port # Q8NES3 Human Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe |
|---|---|
| Gene Symbol | LFNG |
| Gene Full Name | LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
| Background | This gene is a member of the fringe gene family which also includes radical and manic fringe genes. They all encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This gene product is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| Function | Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning (By similarity). [UniProt] |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein. [UniProt] |
| Calculated MW | 42 kDa |
| PTM | A soluble form may be derived from the membrane form by proteolytic processing. [UniProt] |
