ARG22677

anti-Ly-6B.2 antibody [7/4]

anti-Ly-6B.2 antibody [7/4] for IHC-Frozen sections,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

Overview

Product Description Rat Monoclonal antibody [7/4] recognizes Ly-6B.2
This antibody recognizes Ly-6B.2 antigen. Ly-6B.2 is a ~25-30kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosas et al. 2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15kDa (Rosas et al.2010).
In common with other Ly-6 antigens Ly-6B.2 demonstrates a polymorphic expression on inbred mouse strains (Kimura et al. 1984). Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.
T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982).
Rat anti mouse Ly-6B.2 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncello et al. 1991) and the depletion of myeloid cells in vivo (Rosas et al. 2010).
Tested Reactivity Ms
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, WB
Host Rat
Clonality Monoclonal
Clone 7/4
Isotype IgG2a
Target Name Ly-6B.2
Antigen Species Mouse
Immunogen Cultured bone marrow cells
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50 - 1:100
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.