ARG22862

anti-Ly6C antibody [ER-MP20]

anti-Ly6C antibody [ER-MP20] for IHC-Frozen sections,Flow cytometry,Immunoprecipitation and Mouse

Mouse Inflammatory Cell Marker antibody; Neurophil Marker antibody; Mouse MDSC Marker antibody; Myeloid-derived suppressor cell antibody

Overview

Product Description Rat Monoclonal antibody [ER-MP20] recognizes Ly6C
Rat anti Mouse Ly-6C antibody, clone ER-MP20 recognizes murine Ly-6C, a ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenen et al. 1990).
Tested Reactivity Ms
Tested Application FACS, IHC-Fr, IP
Host Rat
Clonality Monoclonal
Clone ER-MP20
Isotype IgG2a
Target Name Ly6C
Antigen Species Mouse
Immunogen Balb/c macrophage precursor cell hybrids.
Conjugation Un-conjugated
Alternate Names AA959465; Ly-6C1; AA682074; Ly-6C; Lymphocyte antigen 6C1; Ly6c

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat
IHC-FrAssay-dependent
IPAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17067 Mouse Ly6c1

Swiss-port # P0CW02 Mouse Lymphocyte antigen 6C1

Gene Symbol Ly6c1
Gene Full Name lymphocyte antigen 6 complex, locus C1
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Research Area Mouse Inflammatory Cell Marker antibody; Neurophil Marker antibody; Mouse MDSC Marker antibody; Myeloid-derived suppressor cell antibody
Calculated MW 14 kDa