ARG54327

anti-MADD antibody

anti-MADD antibody for Western blot and Human,Mouse

Cancer antibody; Cell Death antibody; Metabolism antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes MADD

Tested Reactivity Hu, Ms
Tested Application WB
Specificity This antibody recognizes human and mouse MADD (200-220 kD).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MADD
Antigen Species Human
Immunogen Peptide corresponding to aa 1570-1588 of human MADD. This peptide sequence is identical to that of DENN and differs by one amino acid with rat GDP/GTP exchange protein RAB3-GEP.
Conjugation Un-conjugated
Alternate Names IG20; Differentially expressed in normal and neoplastic cells; DENN; Rab3 GDP/GTP exchange factor; RAB3GEP; Insulinoma glucagonoma clone 20; MAP kinase-activating death domain protein

Application Instructions

Application Note Immunoblotting: use at 1:250 - 1:500 dilution.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa, NIH3T3

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 228355 Mouse MADD

GeneID: 8567 Human MADD

Swiss-port # Q80U28 Mouse MAP kinase-activating death domain protein

Swiss-port # Q8WXG6 Human MAP kinase-activating death domain protein

Gene Symbol MADD
Gene Full Name MAP-kinase activating death domain
Background MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1). Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98% homology with DENN (differentially expressed in neoplasic vs. normal cells) which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greather than 94% homology with a GDO/GTP exchange protein , Rab3-GEP, and it is 87% homologous with KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signalling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.
Function Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect. [UniProt]
Research Area Cancer antibody; Cell Death antibody; Metabolism antibody
Calculated MW 183 kDa