ARG54327
anti-MADD antibody
anti-MADD antibody for Western blot and Human,Mouse
Cancer antibody; Cell Death antibody; Metabolism antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MADD |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | WB |
| Specificity | This antibody recognizes human and mouse MADD (200-220 kD). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MADD |
| Antigen Species | Human |
| Immunogen | Peptide corresponding to aa 1570-1588 of human MADD. This peptide sequence is identical to that of DENN and differs by one amino acid with rat GDP/GTP exchange protein RAB3-GEP. |
| Conjugation | Un-conjugated |
| Alternate Names | IG20; Differentially expressed in normal and neoplastic cells; DENN; Rab3 GDP/GTP exchange factor; RAB3GEP; Insulinoma glucagonoma clone 20; MAP kinase-activating death domain protein |
Application Instructions
| Application Note | Immunoblotting: use at 1:250 - 1:500 dilution. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|---|
| Positive Control | HeLa, NIH3T3 |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunoaffinity chroma-tography |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q80U28 Mouse MAP kinase-activating death domain protein Swiss-port # Q8WXG6 Human MAP kinase-activating death domain protein |
|---|---|
| Gene Symbol | MADD |
| Gene Full Name | MAP-kinase activating death domain |
| Background | MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1). Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98% homology with DENN (differentially expressed in neoplasic vs. normal cells) which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greather than 94% homology with a GDO/GTP exchange protein , Rab3-GEP, and it is 87% homologous with KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signalling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein. |
| Function | Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect. [UniProt] |
| Research Area | Cancer antibody; Cell Death antibody; Metabolism antibody |
| Calculated MW | 183 kDa |
