ARG58035
anti-MAGE1 antibody [MA454]
anti-MAGE1 antibody [MA454] for IHC-Formalin-fixed paraffin-embedded sections and Human,Rat
Overview
| Product Description | Mouse Monoclonal antibody [MA454] recognizes MAGE1 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | IHC-P |
| Specificity | MAGE1 antibody clone MA454 recognizes a protein of 42-46kDa, identified as MAGE-1. This mAb does not cross-react with MAGE-2, -3, -4, -6 -9, -10, or -12 protein. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MA454 |
| Isotype | IgG1, kappa |
| Target Name | MAGE1 |
| Antigen Species | Human |
| Immunogen | Full length Human MAGE1 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | CT1.1; MAGE-1 antigen; MAGE1; Antigen MZ2-E; Melanoma-associated antigen 1; Cancer/testis antigen 1.1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.05% Sodium azide and 0.1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MAGEA1 |
| Gene Full Name | melanoma antigen family A1 |
| Background | This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008] |
| Function | May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. [UniProt] |
| Calculated MW | 34 kDa |
