ARG58035

anti-MAGE1 antibody [MA454]

anti-MAGE1 antibody [MA454] for IHC-Formalin-fixed paraffin-embedded sections and Human,Rat

Overview

Product Description Mouse Monoclonal antibody [MA454] recognizes MAGE1
Tested Reactivity Hu, Rat
Tested Application IHC-P
Specificity MAGE1 antibody clone MA454 recognizes a protein of 42-46kDa, identified as MAGE-1. This mAb does not cross-react with MAGE-2, -3, -4, -6 -9, -10, or -12 protein.
Host Mouse
Clonality Monoclonal
Clone MA454
Isotype IgG1, kappa
Target Name MAGE1
Antigen Species Human
Immunogen Full length Human MAGE1 protein.
Conjugation Un-conjugated
Alternate Names CT1.1; MAGE-1 antigen; MAGE1; Antigen MZ2-E; Melanoma-associated antigen 1; Cancer/testis antigen 1.1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4100 Human MAGEA1

Swiss-port # P43355 Human Melanoma-associated antigen 1

Gene Symbol MAGEA1
Gene Full Name melanoma antigen family A1
Background This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008]
Function May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. [UniProt]
Calculated MW 34 kDa