ARG42894

anti-MAGEC2 antibody

anti-MAGEC2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Flow cytometry,Immunoprecipitation and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes MAGEC2
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MAGEC2
Antigen Species Human
Immunogen Recombinant protein of Human MAGEC2.
Conjugation Un-conjugated
Alternate Names Hepatocellular carcinoma-associated antigen 587; CT10; MAGE-C2 antigen; Cancer/testis antigen 10; MAGEE1; MAGE-E1 antigen; HCA587; Melanoma-associated antigen C2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:20
ICC/IF1:100
IHC-P1:20 - 1:500
IP1:20
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562
Observed Size 55 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 nM Tris-Glycine (pH 7.4), 0.15M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51438 Human MAGEC2

Swiss-port # Q9UBF1 Human Melanoma-associated antigen C2

Gene Symbol MAGEC2
Gene Full Name melanoma antigen family C2
Background This gene is a member of the MAGEC gene family. It is not expressed in normal tissues, except for testis, and is expressed in tumors of various histological types. This gene and the other MAGEC genes are clustered on chromosome Xq26-q27. [provided by RefSeq, Oct 2009]
Function Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination in presence of Ubl-conjugating enzyme UBE2H leading to p53/TP53 degradation. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzymes (E2) at the E3:substrate complex. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Note=Nuclear in germ cells. Cytoplasmic in well-differentiated hepatocellular carcinoma, nuclear in moderately- and poorly-differentiated hepatocellular carcinoma. [UniProt]
Calculated MW 41 kDa