ARG54031

anti-MAP2 antibody (N-term)

anti-MAP2 antibody (N-term) for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse

Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody
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Overview

Product Description Mouse Monoclonal antibody recognizes MAP2
Tested Reactivity Hu, Ms
Tested Application ICC/IF, IP, WB
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Target Name MAP2
Antigen Species Human
Immunogen Purified recombinant human MAP2 (N-terminus) protein fragments expressed in E.coli
Conjugation Un-conjugated
Alternate Names MAP2A; Microtubule-associated protein 2; MAP2C; MAP2B; MAP-2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:150
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 300 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.1M Tris-Glycine (pH 7.4), 150mM NaCl, 0.2%Sodium azide, 0.1%BSA and 50%Glycerol
Preservative 0.2%Sodium azide
Stabilizer 0.1%BSA, 50%Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17756 Mouse MAP2

GeneID: 4133 Human MAP2

Swiss-port # P11137 Human Microtubule-associated protein 2

Swiss-port # P20357 Mouse Microtubule-associated protein 2

Gene Symbol MAP2
Gene Full Name microtubule-associated protein 2
Background The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization.They also seem to have a stiffening effect on microtubules.
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. [UniProt]
Cellular Localization Cytoplasm, cytoskeleton
Highlight Related products:
MAP2 antibodies; MAP2 Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Research Area Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody
Calculated MW 200 kDa
PTM Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).

Customer's Feedback

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Review for anti-MAP2 antibody (N-term)

Application:WB

Sample:Mouse brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC